Delighted staff from the four-star venue were on hand to accept the prestigious title during a gala dinner in Edinburgh on Sunday.
The Conference Hotel started the year on a high after winning the regional title and that victory put them in the running for the national prize.
And on Sunday they overcame stiff competition from a host of other hotels to land the title.
Bronagh Bell, Director of the Golden Jubilee Conference Hotel, commented: “We are very proud that our vision and focus has been recognised yet again by the Scottish Hotel Awards.
"We are passionate about excellence in conference and meeting provision and aim to lead the way in every aspect from food options to delegate health and wellbeing.
“Redeveloping our facilities to provide innovative meeting spaces, access to cutting edge technology, whilst working as an extension of our clients’ teams to deliver well planned events, is what makes us stand out from other Hotels.
"I want to thank every member of our fantastic team for their professionalism and dedication which has made this award possible.”
The title is the latest in a long line of accolades and awards for the Golden Jubilee Conference Hotel, which is celebrating 10 years as a member of Venues of Excellence this year.
Last year they were named Business Hotel of the Year and received the award for Excellence in Hotel Conference Facilities at the Small Business Awards 2017.
They also won the Success Through Sustainability award during the inaugural Working4Business awards and received a TripAdvisor Certificate of Excellence for the seventh year in a row.